Preventing Embedding Resin Bubbles

Improvements in Epoxy Resin Embedding Methods

Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenient embedding methods for electron microscopy. The sections are robust and tissue damage is less than with methacrylate embedding.

Preparation of serial sections of arthropods using 2,2-dimethoxypropane dehydration and epoxy resin embedding under vacuum.

Improved methods are described for anatomical investigation of small insects and other arthropods using serial semithin sections. The specimens were dehydrated with acidified 2,2-dimethoxypropane and embedded in ERL 4206 epoxy resin under vacuum. This procedure ensures good resin impregnation of thin, long body compartments and appendages. Furthermore, it produces excellent overall preservation...

High-refractive index of acrylate embedding resin clarifies mouse brain tissue.

Biological tissue transparency combined with light-sheet fluorescence microscopy is a useful method for studying the neural structure of biological tissues. The development of light-sheet fluorescence microscopy also promotes progress in biological tissue clearing methods. The current clarifying methods mostly use liquid reagent to denature protein or remove lipids first, to eliminate or reduce...

A routine method for embedding animal tissues in Spurr resin for electron microscopy.

The epoxy resin epon 812 has been used in this department to embed a variety of normal and pathological tissues for a number of years. Unfortunately during the past 18 months sectioning qualities of polymerized epon blocks have become variable in an unpredictable manner. It is possible that the batch variation in the quality and composition of the dodecynl succinic anhydride (DDSA) could be res...

Bubbles In Tehran Stock Exchange